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omniscript reverse transcription kit  (Qiagen)


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    Structured Review

    Qiagen omniscript reverse transcription kit
    Omniscript Reverse Transcription Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 98/100, based on 7886 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/omniscript+reverse+transcription+kit/Omniscript+RT+Kit/bio_rxiv__64898__2026__05__08__723137-415-10-14
    Average 98 stars, based on 7886 article reviews
    omniscript reverse transcription kit - by Bioz Stars, 2026-10
    98/100 stars

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    Related Articles

    Synthesized:

    Article Title: Dichotomous SMAD2/3 regulation and selective antihypertrophic activity of heparin during in vitro chondrogenesis of mesenchymal stromal cells
    Article Snippet: RNA was extracted from the homogenate using TRIzol ® reagent (Invitrogen) following the manufacturer’s instructions. .. Complementary DNA was synthesized from 500 ng total RNA using oligo-dT primers and the OmniScript ® Reverse Transcription kit (QIAGEN, Hilden, Germany). .. SYBR ® Green master mix (Thermo Fisher), specific primer sequences (Supplementary Table S1), and a LightCycler ® 96 device (Roche Diagnostics, Basel, Switzerland) were used to determine the transcript levels of the genes of interest via quantitative PCR (qPCR).

    Article Title: Dichotomous SMAD2/3 regulation and selective antihypertrophic activity of heparin during in vitro chondrogenesis of mesenchymal stromal cells.
    Article Snippet: RNA was extracted from the homogenate using TRIzol® reagent (Invitrogen) following the manufacturer’s instructions. .. Complementary DNA was synthesized from 500 ng total RNA using oligo-dT primers and the OmniScript® Reverse Transcription kit (QIAGEN, Hilden, Germany). .. SYBR® Green master mix (Thermo Fisher), specific primer sequences (Supplementary Table S1), and a LightCycler® 96 device (Roche Diagnostics, Basel, Switzerland) were used to determine the transcript levels of the genes of interest via quantitative PCR (qPCR).

    Article Title: Brain region-specific dopamine receptor changes and astrocyte activation influence tau pathology through CDK5 in Alzheimer's disease models.
    Article Snippet: Total RNA was extracted from SH-SY5Y, MC-65, and mouse cortex samples using the RNeasy Mini Kit (Cat# 74104, Qiagen), according to the manufacturer’s protocol. .. Complementary DNA (cDNA) was synthesized using the Omniscript Reverse Transcription Kit (Cat# 205111, Qiagen) with Oligo-dT primers. qPCR was performed using SYBR Green PCR Master Mix (Cat# 4309155, Applied Biosystems, Thermo Fisher Scientific) on a StepOne Real-Time PCR System (Applied Biosystems). ..

    Article Title: Brain region-specific dopamine receptor changes and astrocyte activation influence tau pathology through CDK5 in Alzheimer's disease models
    Article Snippet: Total RNA was extracted from SH-SY5Y, MC-65, and mouse cortex samples using the RNeasy Mini Kit (Cat# 74104, Qiagen), according to the manufacturer's protocol. .. Complementary DNA (cDNA) was synthesized using the Omniscript Reverse Transcription Kit (Cat# 205111, Qiagen) with Oligo-dT primers. qPCR was performed using SYBR Green PCR Master Mix (Cat# 4309155, Applied Biosystems, Thermo Fisher Scientific) on a StepOne Real-Time PCR System (Applied Biosystems). ..

    Reverse Transcription:

    Article Title: Dichotomous SMAD2/3 regulation and selective antihypertrophic activity of heparin during in vitro chondrogenesis of mesenchymal stromal cells
    Article Snippet: RNA was extracted from the homogenate using TRIzol ® reagent (Invitrogen) following the manufacturer’s instructions. .. Complementary DNA was synthesized from 500 ng total RNA using oligo-dT primers and the OmniScript ® Reverse Transcription kit (QIAGEN, Hilden, Germany). .. SYBR ® Green master mix (Thermo Fisher), specific primer sequences (Supplementary Table S1), and a LightCycler ® 96 device (Roche Diagnostics, Basel, Switzerland) were used to determine the transcript levels of the genes of interest via quantitative PCR (qPCR).

    Article Title: Heat-Killed Lacticaseibacillus paracasei HY2782 Attenuates Chronic Kidney Disease by Suppressing Inflammation and Selectively Modulating the Gut Microbial Community.
    Article Snippet: Total RNA was extracted from the kidney and colon tissues using the Easy-spin Total RNA Extraction Kit (iNtRON Biotechnology, Republic of Korea). .. The eluted RNA was reverse transcribed into complementary DNA (cDNA) at 37°C for 1 h using the Omniscript Reverse Transcription Kit (Qiagen, Germany). .. The concentration of the synthesized cDNA was quantified using an Agilent BioTek® Synergy HT Microplate reader (Agilent Technologies, USA).

    Article Title: Dichotomous SMAD2/3 regulation and selective antihypertrophic activity of heparin during in vitro chondrogenesis of mesenchymal stromal cells.
    Article Snippet: RNA was extracted from the homogenate using TRIzol® reagent (Invitrogen) following the manufacturer’s instructions. .. Complementary DNA was synthesized from 500 ng total RNA using oligo-dT primers and the OmniScript® Reverse Transcription kit (QIAGEN, Hilden, Germany). .. SYBR® Green master mix (Thermo Fisher), specific primer sequences (Supplementary Table S1), and a LightCycler® 96 device (Roche Diagnostics, Basel, Switzerland) were used to determine the transcript levels of the genes of interest via quantitative PCR (qPCR).

    Article Title: Brain region-specific dopamine receptor changes and astrocyte activation influence tau pathology through CDK5 in Alzheimer's disease models.
    Article Snippet: Total RNA was extracted from SH-SY5Y, MC-65, and mouse cortex samples using the RNeasy Mini Kit (Cat# 74104, Qiagen), according to the manufacturer’s protocol. .. Complementary DNA (cDNA) was synthesized using the Omniscript Reverse Transcription Kit (Cat# 205111, Qiagen) with Oligo-dT primers. qPCR was performed using SYBR Green PCR Master Mix (Cat# 4309155, Applied Biosystems, Thermo Fisher Scientific) on a StepOne Real-Time PCR System (Applied Biosystems). ..

    Article Title: Anti-Biofilm Activity of Combinations of Cinnamic Acid and Its Derivatives with Cloxacillin Against Methicillin-Resistant Staphylococcus epidermidis
    Article Snippet: .. Reverse transcription was performed using the Omniscript Reverse Transcription Kit (Qiagen, Hilden, Germany) according to the manufacturer’s protocol. .. To the reaction well, 10× RT buffer, dNTP mix, Oligo-dT primer (EURx Ltd., Gdańsk, Poland), Ribonuclease Inhibitor (EURx Ltd., Gdańsk, Poland), and Omniscript Reverse Transcriptase were added.

    Article Title: Brain region-specific dopamine receptor changes and astrocyte activation influence tau pathology through CDK5 in Alzheimer's disease models
    Article Snippet: Total RNA was extracted from SH-SY5Y, MC-65, and mouse cortex samples using the RNeasy Mini Kit (Cat# 74104, Qiagen), according to the manufacturer's protocol. .. Complementary DNA (cDNA) was synthesized using the Omniscript Reverse Transcription Kit (Cat# 205111, Qiagen) with Oligo-dT primers. qPCR was performed using SYBR Green PCR Master Mix (Cat# 4309155, Applied Biosystems, Thermo Fisher Scientific) on a StepOne Real-Time PCR System (Applied Biosystems). ..

    Article Title: Heat-Killed Lacticaseibacillus paracasei HY2782 Attenuates Chronic Kidney Disease by Suppressing Inflammation and Selectively Modulating the Gut Microbial Community
    Article Snippet: Total RNA was extracted from the kidney and colon tissues using the Easy-spin Total RNA Extraction Kit (iNtRON Biotechnology, Republic of Korea). .. The eluted RNA was reverse transcribed into complementary DNA (cDNA) at 37°C for 1 h using the Omniscript Reverse Transcription Kit (Qiagen, Germany). .. The concentration of the synthesized cDNA was quantified using an Agilent BioTek ® Synergy HT Microplate reader (Agilent Technologies, USA).

    Real-time Polymerase Chain Reaction:

    Article Title: Brain region-specific dopamine receptor changes and astrocyte activation influence tau pathology through CDK5 in Alzheimer's disease models.
    Article Snippet: Total RNA was extracted from SH-SY5Y, MC-65, and mouse cortex samples using the RNeasy Mini Kit (Cat# 74104, Qiagen), according to the manufacturer’s protocol. .. Complementary DNA (cDNA) was synthesized using the Omniscript Reverse Transcription Kit (Cat# 205111, Qiagen) with Oligo-dT primers. qPCR was performed using SYBR Green PCR Master Mix (Cat# 4309155, Applied Biosystems, Thermo Fisher Scientific) on a StepOne Real-Time PCR System (Applied Biosystems). ..

    Article Title: Brain region-specific dopamine receptor changes and astrocyte activation influence tau pathology through CDK5 in Alzheimer's disease models
    Article Snippet: Total RNA was extracted from SH-SY5Y, MC-65, and mouse cortex samples using the RNeasy Mini Kit (Cat# 74104, Qiagen), according to the manufacturer's protocol. .. Complementary DNA (cDNA) was synthesized using the Omniscript Reverse Transcription Kit (Cat# 205111, Qiagen) with Oligo-dT primers. qPCR was performed using SYBR Green PCR Master Mix (Cat# 4309155, Applied Biosystems, Thermo Fisher Scientific) on a StepOne Real-Time PCR System (Applied Biosystems). ..

    SYBR Green Assay:

    Article Title: Brain region-specific dopamine receptor changes and astrocyte activation influence tau pathology through CDK5 in Alzheimer's disease models.
    Article Snippet: Total RNA was extracted from SH-SY5Y, MC-65, and mouse cortex samples using the RNeasy Mini Kit (Cat# 74104, Qiagen), according to the manufacturer’s protocol. .. Complementary DNA (cDNA) was synthesized using the Omniscript Reverse Transcription Kit (Cat# 205111, Qiagen) with Oligo-dT primers. qPCR was performed using SYBR Green PCR Master Mix (Cat# 4309155, Applied Biosystems, Thermo Fisher Scientific) on a StepOne Real-Time PCR System (Applied Biosystems). ..

    Article Title: Brain region-specific dopamine receptor changes and astrocyte activation influence tau pathology through CDK5 in Alzheimer's disease models
    Article Snippet: Total RNA was extracted from SH-SY5Y, MC-65, and mouse cortex samples using the RNeasy Mini Kit (Cat# 74104, Qiagen), according to the manufacturer's protocol. .. Complementary DNA (cDNA) was synthesized using the Omniscript Reverse Transcription Kit (Cat# 205111, Qiagen) with Oligo-dT primers. qPCR was performed using SYBR Green PCR Master Mix (Cat# 4309155, Applied Biosystems, Thermo Fisher Scientific) on a StepOne Real-Time PCR System (Applied Biosystems). ..

    Polymerase Chain Reaction:

    Article Title: Brain region-specific dopamine receptor changes and astrocyte activation influence tau pathology through CDK5 in Alzheimer's disease models.
    Article Snippet: Total RNA was extracted from SH-SY5Y, MC-65, and mouse cortex samples using the RNeasy Mini Kit (Cat# 74104, Qiagen), according to the manufacturer’s protocol. .. Complementary DNA (cDNA) was synthesized using the Omniscript Reverse Transcription Kit (Cat# 205111, Qiagen) with Oligo-dT primers. qPCR was performed using SYBR Green PCR Master Mix (Cat# 4309155, Applied Biosystems, Thermo Fisher Scientific) on a StepOne Real-Time PCR System (Applied Biosystems). ..

    Article Title: Brain region-specific dopamine receptor changes and astrocyte activation influence tau pathology through CDK5 in Alzheimer's disease models
    Article Snippet: Total RNA was extracted from SH-SY5Y, MC-65, and mouse cortex samples using the RNeasy Mini Kit (Cat# 74104, Qiagen), according to the manufacturer's protocol. .. Complementary DNA (cDNA) was synthesized using the Omniscript Reverse Transcription Kit (Cat# 205111, Qiagen) with Oligo-dT primers. qPCR was performed using SYBR Green PCR Master Mix (Cat# 4309155, Applied Biosystems, Thermo Fisher Scientific) on a StepOne Real-Time PCR System (Applied Biosystems). ..

    other:

    Article Title: Natural aging drives a subclinical cardiovascular phenotype in a non-human primate
    Article Snippet: RNA quantification and quality control was performed by spectrophotometry (NanoDrop – Thermo Scientific); guidelines regarding wave length absorbance ratio of 260/280 and 260/230 were followed, so that the ratios between the wave lengths 230:260:280 of 1:2:1 were accepted.



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